AI / Methods2 days agoNew
Engineering editopes through programmable RNA editing toward tumor neoantigen generation.
Researchers developed SPEAR gRNAs to harness endogenous ADAR1 for precise A-to-I RNA editing, creating immunogenic neoepitopes termed 'editopes.' In a melanoma model using the MART-1 antigen, this approach restored T-cell recognition and enabled tumor control in vivo. The team also established a computational pipeline to identify candidate tumor-selective neoepitopes across multiple cancer types.