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Glossary · concept

Antibody epitope

The specific surface patch on an antigen that an antibody binds — often a folded shape, not the short HLA-presented peptide seen by T cells.

An antibody epitope is the exact part of an antigen recognized by an antibody. It can be linear, meaning a continuous stretch of amino acids, or conformational, meaning a three-dimensional surface made by residues that are far apart in sequence but close together in the folded protein.

This is different from the peptide epitopes used in most neoantigen-vaccine pipelines. T-cell epitopes are short peptides presented by HLA molecules; antibody epitopes are exposed surfaces on intact proteins. The prediction problems, assays, and therapeutic uses are therefore different.

Antibody-epitope prediction still matters to the field because cancer vaccines, bispecifics, ADCs, and immune monitoring all ask related targeting questions: which molecular surface is visible, specific, and safe enough to aim an immune response at?

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What is the difference between an antibody epitope and a T-cell epitope?

An antibody epitope is a surface feature on an intact antigen that an antibody binds directly. A T-cell epitope is usually a short peptide displayed by HLA/MHC and recognized by a T-cell receptor. Neoantigen vaccines mostly target T-cell epitopes, not antibody epitopes.

Are antibody epitopes always linear sequences?

No. Some are linear stretches of amino acids, but many important antibody epitopes are conformational surfaces formed by the folded 3D structure of a protein.