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Clinical
Identification of pre-existing ubiquitous neoantigen-reactive tumor-infiltrating T-cells in a patient with metastatic pancreatic neuroendocrine tumor
A preprint investigates whether ubiquitous neoantigens—mutations shared across all metastatic sites—can overcome inter-site heterogeneity in pancreatic neuroendocrine tumors. Using whole-exome and RNA sequencing on 14 samples from a treatment-naive patient, researchers identified ubiquitous mutations and validated immunogenicity via IFN-gamma ELISpot and single-cell TCR sequencing. This approach targets durable immune responses by focusing on neoantigens present in both primary and metastatic lesions.